Hepatitis B virus genotypes in Southeast Brazil and its relationship with histological features|
Nabuco, Leticia Cancella; Mello, Francisco Campello do Amaral; Gomes, Selma de Andrade; Perez, Renata Mello; Soares, Jorge André Segadas; Coelho, Henrique Sérgio Moraes & Nogueira, Cristiane Alves Villela
Data concerning the relationship between hepatitis B virus (HBV) genotypes and liver histology are scarce.
The aim of this study was to compare HBV non-B and non-C genotypes according to demographic features, clinical
status, HBV-DNA levels and liver histology in Rio de Janeiro. One hundred twenty one consecutive chronic HBVinfected
patients were enrolled during two-year period and data were prospectively collected. Sera were tested for
HBV genotyping using restriction fragment length polymorphism. Liver biopsy was obtained from patients with
either increased alanine aminotransferase (ALT) or HBV-DNA levels. Genotype A was the most common, found in
82 (68%) patients, followed by F in 19 (15%), D in 17 (14%), B in one (1%) and C in two (2%). There was no association
between HBV genotypes A, D and F and gender (p = 0.37), age (p = 0.78), race (p = 0.22), mode of infection (p
= 0.94), HB "e" antigen status (p = 0.37) and HBV-DNA levels (p = 0.47). The ALT levels were lower in genotype D
(75%) compared with A (47%) and F (55%) (p = 0.05). Liver biopsy showed lower inflammation [histological activity
index (HAI) = 4] and fibrosis (F) (= 0) scores in genotype D than in genotypes A (HAI = 5, p < 0.001; F = 2, p
= 0.008) or F (HAI = 5, p = 0.009; F = 2, p = 0.01). Genotype A was the most prevalent in chronic HBV-infected
patients and genotype D patients presented with less intense liver disease.
HBV genotypes - hepatitis B - HBV-DNA - liver histology - HBeAg - ALT