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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 8, 2012, pp. 969-970
Bioline Code: oc12161
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 8, 2012, pp. 969-970

 en Rapid tests for the detection of the Mycobacterium abscessus check for this species in other resources subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil
Matsumoto, Cristianne Kayoko; Chimara, Erica; Ramos, Jesus Pais; Campos, Carlos Eduardo Dias; Caldas, Paulo Cesar de Souza; Lima, Karla Valeria Batista; Lopes, Maria Luiza; Duarte, Rafael Silva & Leão, Sylvia Cardoso

Abstract

A single strain of Mycobacterium abscessus check for this species in other resources subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain.

Keywords
Mycobacterium abscessus - epidemic - rapid test - rpoB - epidemiological surveillance

 
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