search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 8, 2012, pp. 978-984
Bioline Code: oc12162
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 8, 2012, pp. 978-984

 en In vitro antimicrobial assessment of Cuban propolis extracts
Monzote, Lianet; Cuesta-Rubio, Osmany; Fernandez, Mercedes Campo; Hernandez, Ingrid Márquez; Fraga, Jorge; Pérez, Kleich; Kerstens, Monique; Maes, Louis & Cos, Paul

Abstract

Propolis is a resinous mixture of different plant exudates collected by honeybees. Currently, propolis is widely used as a food supplement and in folk medicine. We have evaluated 20 Cuban propolis extracts of different chemical types, brown (BCP), red and yellow (YCP), with respect to their in vitro antibacterial, antifungal and antiprotozoal properties. The extracts inhibited the growth of Staphylococcus aureus check for this species in other resources and Trichophyton rubrum check for this species in other resources at low μg/mL concentrations, whereas they were not active against Escherichia coli check for this species in other resources and Candida albicans check for this species in other resources . The major activity of the extracts was found against the protozoa Leishmania check for this species in other resources , Trypanosoma check for this species in other resources and Plasmodium check for this species in other resources , although cytotoxicity against MRC-5 cells was also observed. The BCP-3, YCP-39 and YCP-60 extracts showed the highest activity against P. falciparum check for this species in other resources , with 50% of microbial growth (IC50) values of 0.2 μg/mL. A positive correlation between the biological activity and the chemical composition was observed for YCP extracts. The most promising antimicrobial activity corresponds to YCP subtype B, which contains acetyl triterpenes as the main constituents. The present in vitro study highlights the potential of propolis against protozoa, but further research is needed to increase selectivity towards the parasite. The observed chemical composition-activity relationship of propolis can contribute to the identification of the active principles and standardisation of this bee product.

Keywords
propolis - Cuban - antibacterial - antifungal - antiprotozoal

 
© Copyright 2012 - Memorias do Instituto Oswaldo Cruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil