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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 8, 2012, pp. 1073-1075
Bioline Code: oc12179
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 8, 2012, pp. 1073-1075

 en Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
Melandri, Vanessa; Guimarães, Anthony Érico; Komar, Nicholas; Nogueira, Maurício L; Mondini, Adriano; Fernandez-Sesma, Ana; Alencar, Jeronimo & Bosch, Irene

Abstract

In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.

Keywords
WNV - b-ELISA - PRNT - Brazil

 
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