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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 7, 2012, pp. 946-950
Bioline Code: oc12199
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 7, 2012, pp. 946-950

 en Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli check for this species in other resources and Trypanosoma cruzi check for this species in other resources
Jobe, Momodou; Anwuzia-Iwegbu, Charles; Banful, Ama; Bosier, Emma; Iqbal, Mubeen; Jones, Kelly; Lecutier, Suzanne J; Lepper, Kasimir; Redmond, Matt; Ross-Parker, Andrew; Ward, Emily; Wernham, Paul; Whidden, Eleanor M; Tyler, Kevin M & Steverding, Dietmar

Abstract

In this study the effect of eight DNA topoisomerase inhibitors on the growth Trypanosoma rangeli check for this species in other resources epimastigotes in cell culture was investigated. Among the eight compounds tested, idarubicin was the only compound that displayed promising trypanocidal activity with a half-maximal growth inhibition (GI50) value in the sub-micromolar range. Fluorescence-activated cell sorting analysis showed a reduction in DNA content in T. rangeli epimastigotes when treated with idarubicin. In contrast to T. rangeli, against Trypanosoma cruzi check for this species in other resources epimastigotes idarubicin was much less effective exhibiting a GI50 value in the mid-micromolar range. This result indicates that idarubicin dis- plays differential toxic effects in T. rangeli and T. cruzi. Compared with African trypanosomes, it seems that American trypanosomes are generally less susceptible to DNA topoisomerase inhibitors.

Keywords
Trypanosoma rangeli - Trypanosoma cruzi - Chagas disease - DNA topoisomerase inhibitors - drug screening - chemotherapy

 
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