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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 7, 2012, pp. 960-963
Bioline Code: oc12203
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 7, 2012, pp. 960-963

 en An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y
dos Santos da Silva, Alexandre; de Vasconcelosa, Gentil Arthur Lins Bentes Mendonça; Kappel, Livia Abud; Pinto, Marcelo Alves & de Paula, Vanessa Salete

Abstract

The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of dis- ease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive im- munoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our “in-house” assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed “in house” could be used as an alternative to commercial assays that utilise IgG.

Keywords
IgY - hepatitis A - ELISA

 
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