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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 1, 2013, pp. 65-72
Bioline Code: oc13011
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 1, 2013, pp. 65-72

 en Extended genetic analysis of Brazilian isolates of Bacillus cereus check for this species in other resources and Bacillus thuringiensis check for this species in other resources
Zahner, Viviane; e Silva, Ana Carolina Telles de Carvalho; de Moraes, Gabriela Pinhel; McIntosh, Douglas & de Filippis, Ivano

Abstract

Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus check for this species in other resources and Bacillus thuringiensis check for this species in other resources previously characterized in terms of presence/absence of sequences encoding virulence factors and via/ variable number tandem repeat (VNTR). Additional analysis involved polymerase chain reaction for the presence of sequences (be, cytK, inA, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. MLST analysis ascribed novel and unique sequence types to each of the isolates. A phylogenetic tree was constructed from a single sequence of 2,838 bp of concatenated loci sequences. The strains were not monophyletic by analysis of any specific housekeeping gene or virulence characteristic. No clear association in relation to source of isolation or to genotypic profile based on the presence or absence of putative virulence genes could be identified. Comparison of VNTR profiling with MLST data suggested a correlation between these two methods of genetic analysis. In common with the majority of previous studies, MLST was unable to provide clarification of the basis for pathogenicity among members of the B. cereus complex. Nevertheless, our application of MLST served to reinforce the notion that B. cereus and B. thuringiensis should be considered as the same species.

Keywords
Bacillus cereus - multiple locus sequence typing - virulence factors

 
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