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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 1, 2013, pp. 116-118
Bioline Code: oc13020
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 1, 2013, pp. 116-118

 en Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis check for this species in other resources (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients
Eamsobhana, Praphathip; Wanachiwanawin, Darawan; Dechkum, Naowarat; Parsartvit, Anchana & Yong, Hoi Sen

Abstract

Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus cantonensis check for this species in other resources -specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-an- tibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. The PCR method detected A. cantonensis DNA in CSF samples from four of 10 serologically confirmed angiostrongyliasis cases. The PCR results were negative for the remaining CSF samples. The nucleotide sequences of three positive CSF-PCR samples shared 98.8-99.2% similarity with the reference sequence of A. cantonensis. These results indicate the potential application of this PCR assay with clinical CSF samples for additional support in the confirmation of eosinophilic meningitis due to A. cantonensis.

Keywords
Angiostrongylus cantonensis - eosinophilic meningitis/meningoencephalitis - cerebrospinal fluid -PCR - DNA sequencing

 
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