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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 2, 2013, pp. 131-139
Bioline Code: oc13025
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 2, 2013, pp. 131-139

 en Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis check for this species in other resources for the diagnosis of tuberculosis infection
Araujo, Zaida; Giampietro, Francesca; de los Angeles Bochichio, María; Palacios, Andrea; Dinis, Jenifer; Isern, Jaime; Henry de Waard, Jacobus; Rada, Elsa; Borges, Rafael; Fernández de Larrea, Carlos; Villasmil, Angel; Vanegas, Magnolia; Enciso-Moreno, Jose Antonio & Patarroyo, Manuel Alfonso

Abstract

The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis check for this species in other resources ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.

Keywords
pulmonary tuberculosis; extrapulmonary tuberculosis; synthetic peptide; ESAT-6 antigen; Ag85A antigen

 
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