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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 2, 2013, pp. 167-171
Bioline Code: oc13031
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 2, 2013, pp. 167-171

 en Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens
Guzmán, Camilo; Mattar, Salim; Levis, Silvana; Pini, Noemí; Figueiredo, Tadeu; Mills, James & Salazar-Bravo, Jorge

Abstract

We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus (ARAV) as antigens for the detection of antibodies against hantaviruses. Twenty-four human sera were IgG positive using one or both antigens. We detected anti-MACV IgG antibodies in 10 sera (3.5%) and anti-ARAV antibodies in 21 sera (7.34%). Of the 10 samples that were positive for MACV, seven (70%) were cross-reactive with ARAV; seven of the 21 ARAV-positive samples were cross-reactive with MACV. Using an ARAV IgM ELISA, two of the 24 human sera (8.4%) were positive. We captured 322 rodents, including 210 Cricetidae (181 Zygodontomys brevicauda check for this species in other resources , 28 Oligoryzomys fulvescens check for this species in other resources and 1 Oecomys trinitatis check for this species in other resources ), six Heteromys anomalus check for this species in other resources (Heteromyidae), one Proechimys check for this species in other resources sp. (Echimyidae) and 105 Muridae (34 Rattus rattus check for this species in other resources and 71 Mus musculus check for this species in other resources ). All rodent sera were negative for both antigens. The 8.4% detection rate of hantavirus antibodies in humans is much higher than previously found in serosurveys in North America, suggesting that rural agricultural workers in northeastern Colombia are frequently exposed to hantaviruses. Our results also indicate that tests conducted with South American hantavirus antigens could have predictive value and could represent a useful alternative for the diagnosis of hantavirus infection in Colombia.

Keywords
hantavirus; Colombia; epidemiological surveillance; Rodentia; public health; immunoassay

 
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