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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 2, 2013, pp. 246-247
Bioline Code: oc13044
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 2, 2013, pp. 246-247

 en Use of sodC versus ctrA for real-time polymerase chain reaction-based detection of Neisseria meningitidis check for this species in other resources in sterile body fluids
Higa, Fábio Takenori; Fukasawa, Lucila Okuyama; Gonçalves, Maria Gisele; Salgado, Maristela Marques; Silva de Lemos, Ana Paula; Harrison, Lee H; Lima de Oliveira, Priscilla; Naufal da Silva, Carla & Sacchi, Claudio Tavares

Abstract

We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis check for this species in other resources in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.

Keywords
sodC-based RT-PCR; N. meningitidis diagnostics; meningitis

 
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