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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 3, 2013, pp. 272-279
Bioline Code: oc13050
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 3, 2013, pp. 272-279

 en Multiplex polymerase chain reaction to identify and determine the toxigenicity of Corynebacterium check for this species in other resources spp with zoonotic potential and an overview of human and animal infections
Costa Torres, Luciene de Fátima; Ribeiro, Dayana; Hirata Jr, Raphael; Carvalho Pacheco, Luis Gustavo; Souza, Monica Cristina; dos Santos, Louisy Sanches; dos Santos, Cíntia Silva; Salah, Mohammad; da Costa, Mateus Matiuzzi; Ribeiro, Marcio Garcia; Selim, Salah A.; de Carvalho Azevedo, Vasco Ariston & Mattos-Guaraldi, Ana Luiza

Abstract

Corynebacterium diphtheriae check for this species in other resources , Corynebacterium ulcerans check for this species in other resources and Corynebacterium pseudotuberculosis check for this species in other resources constitute a group of potentially toxigenic microorganisms that are related to different infectious processes in animal and human hosts. Currently, there is a lack of information on the prevalence of disease caused by these pathogens, which is partially due to a reduction in the frequency of routine laboratory testing. In this study, a multiplex polymerase chain reaction (mPCR) assay that can simultaneously identify and determine the toxigenicity of these corynebacterial species with zoonotic potential was developed. This assay uses five primer pairs targeting the following genes: rpoB (Corynebacterium spp), 16S rRNA (C. ulcerans and C. pseudotuberculosis), pld (C. pseudotuberculosis), dtxR (C. diphtheriae) and tox [diphtheria toxin (DT)]. In addition to describing this assay, we review the literature regarding the diseases caused by these pathogens. Of the 213 coryneform strains tested, the mPCR results for all toxigenic and non-toxigenic strains of C. diphtheriae, C. ulcerans and C. pseudotuberculosis were in 100% agreement with the results of standard biochemical tests and PCR-DT. As an alternative to conventional methods, due to its advantages of specificity and speed, the mPCR assay used in this study may successfully be applied for the diagnosis of human and/or animal diseases caused by potentially toxigenic corynebacterial species.

Keywords
mPCR; C. diphtheriae; C. ulcerans; C. pseudotuberculosis; diphtheria toxin

 
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