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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 3, 2013, pp. 312-320
Bioline Code: oc13056
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 3, 2013, pp. 312-320

 en Genetic compatibility between Anopheles lesteri check for this species in other resources from Korea and Anopheles paraliae check for this species in other resources from Thailand
Taai, Kritsana; Baimai, Visut; Saeung, Atiporn; Thongsahuan, Sorawat; Min, Gi-Sik; Otsuka, Yasushi; Park, Mi-Hyun; Fukuda, Masako; Somboon, Pradya & Choochote, Wej

Abstract

To assess differentiation and relationships between Anopheles lesteri check for this species in other resources and Anopheles paraliae check for this species in other resources we established three and five iso-female lines of An. lesteri from Korea and An. paraliae from Thailand, respectively. These isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing DNA sequences of ribosomal DNA second internal transcribed spacer (ITS2) and mitochondrial DNA cytochrome c oxidase subunit I (COI) and subunit II (COII). Results of reciprocal and F1-hybrid crosses between An. lesteri and An. paraliae indicated that they were compatible genetically producing viable progenies and complete synaptic salivary gland polytene chromosomes without inversion loops in all chromosome arms. The pairwise genetic distances of ITS2, COI and COII between these morphological species were 0.040, 0.007-0.017 and 0.008-0.011, respectively. The specific species status of An. paraliae in Thailand and/or other parts of the continent are discussed.

Keywords
Anopheles lesteri; Anopheles paraliae; crossing experiments; second internal transcribed spacer; cytochrome c oxidase subunit I; cytochrome c oxidase subunit II

 
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