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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 3, 2013, pp. 390-391
Bioline Code: oc13067
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 3, 2013, pp. 390-391

 en A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia check for this species in other resources
Gallo, Stephanie Wagner; Ramos, Patrícia Locosque; Ferreira, Carlos Alexandre Sanchez & Dias de Oliveira, Sílvia

Abstract

Stenotrophomonas maltophilia check for this species in other resources is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.

Keywords
Stenotrophomonas maltophilia; identification; PCR

 
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