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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 4, 2013, pp. 408-413
Bioline Code: oc13071
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 4, 2013, pp. 408-413

 en Induction of protective T-helper 1 immune responses against Echinococcus granulosus check for this species in other resources in mice by a multi-T-cell epitope antigen based on five proteins
Esmaelizad, Majid; Ahmadian, Gholamreza; Aghaiypour, Khosrow; Shamsara, Mehdi; Paykari, Habibellah & Tebianian, Majid

Abstract

In this study, we designed an experiment to predict a potential immunodominant T-cell epitope and evaluate the protectivity of this antigen in immunised mice. The T-cell epitopes of the candidate proteins (EgGST, EgA31, Eg95, EgTrp and P14-3-3) were detected using available web-based databases. The synthesised DNA was subcloned into the pET41a+ vector and expressed in Escherichia coli check for this species in other resources as a fusion to glutathione-S-transferase protein (GST). The resulting chimeric protein was then purified by affinity chromatography. Twenty female C57BL/6 mice were immunised with the antigen emulsified in Freund’s adjuvant. Mouse splenocytes were then cultured in Dulbecco’s Modified Eagle’s Medium in the presence of the antigen. The production of interferon-γ was significantly higher in the immunised mice than in the control mice (> 1,300 pg/mL), but interleukin (IL)-10 and IL-4 production was not statistically different between the two groups. In a challenge study in which mice were infected with 500 live protoscolices, a high protectivity level (99.6%) was demonstrated in immunised BALB/C mice compared to the findings in the control groups [GST and adjuvant (Adj)]. These results demonstrate the successful application of the predicted T-cell epitope in designing a vaccine against Echinococcus granulosus check for this species in other resources in a mouse model.

Keywords
multi-epitope; vaccine; EgGST; Eg95; EgA31; EgTrp

 
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