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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 4, 2013, pp. 414-420
Bioline Code: oc13072
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 4, 2013, pp. 414-420

 en Detection of Wolbachia pipientis check for this species in other resources , including a new strain containing the wsp gene, in two sister species of Paraphlebotomus check for this species in other resources sandflies, potential vectors of zoonotic cutaneous leishmaniasis
Parvizi, Parviz; Bordbar, Ali & Najafzadeh, Narmin

Abstract

Individual, naturally occurring Phlebotomus mongolensis check for this species in other resources and Phlebotomus caucasicus check for this species in other resources from Iran were screened for infections with the maternally inherited intracellular Rickettsia-like bacterium Wolbachia pipientis check for this species in other resources via targeting a major surface protein gene (wsp). The main objective of this study was to determine if W. pipientis could be detected in these species. The sandflies were screened using polymerase chain reaction to amplify a fragment of the Wolbachia surface protein gene. The obtained sequences were edited and aligned with database sequences to identify W. pipientis haplotypes. Two strains of Wolbachia were found. Strain Turk 54 (accession EU780683) is widespread and has previously been reported in Phlebotomus papatasi check for this species in other resources and other insects. Strain Turk 07 (accession KC576916) is a novel strain, found for first time in the two sister species. A-group strains of W. pipientis occur throughout much of the habitat of these sandflies. It is possible that Wolbachia is transferred via horizontal transmission. Horizontal transfer could shed light on sandfly control because Wolbachia is believed to drive a deleterious gene into sandflies that reduces their natural population density. With regard to our findings in this study, we can conclude that one species of sandfly can be infected with different Wolbachia strains and that different species of sandflies can be infected with a common strain.

Keywords
Wolbachia pipientis; wsp gene; Paraphlebotomus; L. major; Iran

 
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