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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 4, 2013, pp. 457-462
Bioline Code: oc13078
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 4, 2013, pp. 457-462

 en Nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of Rondônia, Brazil
de Lima, Cleoni Alves Mendes; Gomes, Harrison Magdinier; Oelemann, Maraníbia Aparecida Cardoso; Ramos, Jesus Pais; Caldas, Paulo Cezar; Campos, Carlos Eduardo Dias; Pereira, Márcia Aparecida da Silva; Montes, Fátima Fandinho Onofre; de Oliveira, Maria do Socorro Calixto; Suffys, Philip Noel & da Fonseca Moura, Maria Manuela

Abstract

The main cause of pulmonary tuberculosis (TB) is infection with Mycobacterium tuberculosis check for this species in other resources (MTB). We aimed to evaluate the contribution of nontuberculous mycobacteria (NTM) to pulmonary disease in patients from the state of Rondônia using respiratory samples and epidemiological data from TB cases. Mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. Among the 1,812 cases suspected of having pulmonary TB, 444 yielded bacterial cultures, including 369 cases positive for MTB and 75 cases positive for NTM. Within the latter group, 14 species were identified as Mycobacterium abscessus check for this species in other resources , Mycobacterium avium check for this species in other resources , Mycobacterium fortuitum check for this species in other resources , Mycobacterium intracellulare check for this species in other resources , Mycobacterium gilvum check for this species in other resources , Mycobacterium gordonae check for this species in other resources , Mycobacterium asiaticum check for this species in other resources , Mycobacterium tusciae check for this species in other resources , Mycobacterium porcinum check for this species in other resources , Mycobacterium novocastrense check for this species in other resources , Mycobacterium simiae check for this species in other resources , Mycobacterium szulgai check for this species in other resources , Mycobacterium phlei check for this species in other resources and Mycobacterium holsaticum check for this species in other resources and 13 isolates could not be identified at the species level. The majority of NTM cases were observed in Porto Velho and the relative frequency of NTM compared with MTB was highest in Ji-Paraná. In approximately half of the TB subjects with NTM, a second sample containing NTM was obtained, confirming this as the disease-causing agent. The most frequently observed NTM species were M. abscessus and M. avium and because the former species is resistant to many antibiotics and displays unsatisfactory cure rates, the implementation of rapid identification of mycobacterium species is of considerable importance.

Keywords
pulmonary tuberculosis; nontuberculous mycobacteria; PRA; Rondônia; Brazil

 
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