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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 5, 2013, pp. 000-000
Bioline Code: oc13092
Full paper language: English
Document type: Note
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 5, 2013, pp. 000-000

 en Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans check for this species in other resources serovar Canicola
Shimabukuro, Fabio Hiroto; da Costa, Veruska Maia; da Silva, Rodrigo Costa; Langoni, Hélio; da Silva, Aristeu Vieira; de Carvalho, Lídia Raquel & Domingues, Paulo Francisco

Abstract

Mice experimentally infected with a pathogenic strain of Leptospira interrogans check for this species in other resources serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.

Keywords
microscopic agglutination test; prozone effect; leptospirosis

 
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