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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 5, 2013, pp. 631-636
Bioline Code: oc13108
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 5, 2013, pp. 631-636

 en Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
Souza, Ligia Cristina Kalb; Gonçalves Pinho, Rosana Elisa Gonçalves; Lima, Carla Vanessa de Paula; Fragoso, Stênio Perdigão & Soares, Maurilio José

Abstract

Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi check for this species in other resources actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei check for this species in other resources and Leishmania major check for this species in other resources ), insects ( Angomonas deanei check for this species in other resources , Crithidia fasciculata check for this species in other resources , Herpetomonas samuelpessoai check for this species in other resources and Strigomonas culicis check for this species in other resources ) and plants ( Phytomonas serpens check for this species in other resources ). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.

Keywords
actin; confocal microscopy; trypanosomatids; Trypanosoma cruzi

 
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