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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 6, 2013, pp. 724-729
Bioline Code: oc13121
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 6, 2013, pp. 724-729

 en Antagonistic activity expressed by Shigella sonnei check for this species in other resources : identification of a putative new bacteriocin
Bernardes Sousa, Mireille Ângela; de Macêdo Farias, Luiz; Luciana de Oliveira, Patrícia; Silvana Moreira, Jaqueline; Morais Apolônio, Ana Carolina; Silvano Oliveira, Jamil; Matos Santoro, Marcelo; Nogueira Mendes, Edilberto & Prazeres Magalhães, Paula

Abstract

Bacteriocins are antibacterial, proteinaceous substances that mediate microbial dynamics. Bacteriocin production is a highly disseminated property among all major lineages of bacteria, including Shigella check for this species in other resources . In this paper, we addressed the purification and characterisation of a bacteriocin produced by a Shigella sonnei check for this species in other resources strain (SS9) isolated from a child with acute diarrhoea. The substance was purified through ammonium-sulphate precipitation and sequential steps of chromatography. The intracellular fraction obtained at 75% ammonium sulphate maintained activity following exposure to pH values from 1-11 and storage at -80ºC for more than two years and was inactivated by high temperatures and proteases. The molecular mass of the purified bacteriocin was determined by mass spectrometry to be 18.56 kDa. The N-terminal sequence of the bacteriocin did not match any other antibacterial proteins described. A putative new bacteriocin produced by S. sonnei has been detected. This bacteriocin may represent a newly described protein or a previously described protein with a newly detected function. Considering that SS9 expresses antagonism against other diarrhoeagenic bacteria, the bacteriocin may contribute to S. sonnei virulence and is potentially applicable to either preventing or controlling diarrhoeal disease.

Keywords
Shigella sonnei; bacteriocin; colicin; antagonistic substance; acute diarrhoea

 
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