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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 7, 2013, pp. 836-844
Bioline Code: oc13141
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 7, 2013, pp. 836-844

 en Interaction of lipophorin with Rhodnius prolixus check for this species in other resources oocytes: biochemical properties and the importance of blood feeding
Entringer, Petter Franco; Grillo, Luciano Aparecido Meireles; Pontes, Emerson Guedes; Machado, Ednildo Alcântara & Gondim, Katia Calp

Abstract

Lipophorin (Lp) is the main haemolymphatic lipoprotein in insects and transports lipids between different organs. In adult females, lipophorin delivers lipids to growing oocytes. In this study, the interaction of this lipoprotein with the ovaries of Rhodnius prolixus check for this species in other resources was characterised using an oocyte membrane preparation and purified radiolabelled Lp (125I-Lp). Lp-specific binding to the oocyte membrane reached equilibrium after 40-60 min and when 125I-Lp was incubated with increasing amounts of membrane protein, corresponding increases in Lp binding were observed. The specific binding of Lp to the membrane preparation was a saturable process, with a Kd of 7.1 ± 0.9 x 10-8 M and a maximal binding capacity of 430 ± 40 ng 125I-Lp/μg of membrane protein. The binding was calcium independent and pH sensitive, reaching its maximum at pH 5.2-5.7. Suramin inhibited the binding interaction between Lp and the oocyte membranes, which was completely abolished at 0.5 mM suramin. The oocyte membrane preparation from R. prolixus also showed binding to Lp from Manduca sexta check for this species in other resources . When Lp was fluorescently labelled and injected into vitellogenic females, the level of Lp-oocyte binding was much higher in females that were fed whole blood than in those fed blood plasma.

Keywords
haematophagous insect; lipophorin receptor; ovary; oocyte; Rhodnius prolixus

 
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