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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 7, 2013, pp. 909-913
Bioline Code: oc13151
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 7, 2013, pp. 909-913

 en A new survey of the serology of human Trypanosoma cruzi check for this species in other resources infection in the Rio Negro microregion, Brazilian Amazon: a critical analysis
Coura, José Rodrigues; Marquez, Maurício Humberto Peña; de Oliveira Guerra, Jorge Augusto; Zauza, Patricia Lago; Miguel, Julio Cesar & Pereira, José Borges

Abstract

The serology of human Trypanosoma cruzi check for this species in other resources infection in the Rio Negro microregion is very complex because of the large numbers of false-positive cases that result from low antibody titres and cross-reactions with other infections. In the present study, we collected 4,880 blood samples on filter paper; of these, indirect immunofluorescence (IIF) was strongly reactive in 221 (4.5%), which were considered to be positive (IIF strongly reactive; high intensity of fluorescence) and weakly reactive in 302 (6.2%), which were considered to be doubtful (IIF weakly reactive; low intensity of fluorescence). The confirmatory test on the serum using at least two of three techniques (IIF, conventional ELISA and recombinant ELISA) on 137 samples that were positive in the screening test only confirmed 33 cases (24.1%). Of the 178 samples that were considered doubtful in the screening test, only 10 (5.6%) were considered to be positive in the confirmatory test. Finally, we recommend that the serological diagnosis of T. cruzi infection in the Amazon region be made using at least two different techniques, for example immunofluorescence and ELISA and confirmed by Western blot analysis when possible.

Keywords
Trypanosoma cruzi check for this species in other resources ; human infection; serology; Brazilian Amazon

 
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