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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 7, 2013, pp. 928-931
Bioline Code: oc13155
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 7, 2013, pp. 928-931

 en Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi check for this species in other resources infection in a nonendemic setting
Izquierdo, Luis; Marques, Alexandre Ferreira; Gαllego, Montserrat; Sanz, Sílvia; Tebar, Sílvia; Riera, Cristina; Quintó, Llorenç; Aldasoro, Edelweiss; Almeida, Igor C. & Gascon, Joaquim

Abstract

The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi check for this species in other resources was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive [100%; 95% confidence interval (CI) = 96-100] and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment

Keywords
Chagas disease; lytic anti-α-Gal antibodies; Trypanosoma cruzi

 
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