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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 7, 2013, pp. 932-935
Bioline Code: oc13156
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 7, 2013, pp. 932-935

 en The identification of two Trypanosoma cruzi check for this species in other resources I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region
Villa, Lina Marcela; Guhl, Felipe; Zabala, Daniel; Ramírez, Juan David; Urrea, Daniel Alfonso; Hernαndez, Diana Carolina; Cucunubα, Zulma; Montilla, Marleny; Carranza, Julio César & Rueda, Karina


A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi check for this species in other resources I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruzi I to distinguish TcIDOM from sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.

spliced-leader intergenic region; Trypanosoma cruzi I; PCR; Chagas disease; domestic cycle; sylvatic cycle

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