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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 8, 2013, pp. 1074-1077
Bioline Code: oc13179
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 8, 2013, pp. 1074-1077

 en Cell vacuolation induced by Haemophilus influenzae check for this species in other resources supernatants in HEp-2 cells
Espinoza-Mellado, María del Rosario; López-Villegas, Edgar Oliver; Arteaga-Garibay, Ramón I & Giono-Cerezo, Silvia

Abstract

Haemophilus influenzae check for this species in other resources belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a “vacuolating factor” in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.

Keywords
Haemophilus influenzae; cell vacuolation; cytotoxicity assays

 
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