search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. s1, 2013, pp. 110-122
Bioline Code: oc13195
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. s1, 2013, pp. 110-122

 en COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil
Laurito, Magdalena; de Oliveira, Tatiane M.P.; Almirón, Walter Ricardo & Sallum, Maria Anice Mureb

Abstract

Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus check for this species in other resources , Cx. chidesteri check for this species in other resources , Cx. dolosus check for this species in other resources , Cx. lygrus check for this species in other resources and Cx. saltanensis check for this species in other resources ) with intraspecific divergences lower than 1.75%. Cx. bilineatus check for this species in other resources is formally resurrected from the synonymy of Cx. dolosus. Cx. maxi check for this species in other resources , Cx. surinamensis check for this species in other resources and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens check for this species in other resources (3%) was almost twice the interspecific between it and Cx. quinquefasciatus check for this species in other resources (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex (Cux.) species.

Keywords
Culex; identification; COI; morphology; genetic divergence

 
© Copyright 2013 - Memórias do Instituto Oswaldo Cruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil