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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 2, 2014, pp. 236-245
Bioline Code: oc14032
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 2, 2014, pp. 236-245

 en Genotyping Mycobacterium bovis check for this species in other resources from cattle in the Central Pampas of Argentina: temporal and regional trends
Shimizu, Ernesto; Macías, Analía; Paolicchi, Fernando; Magnano, Gabriel; Zapata, Laura; Fernàndez, Analía; Canal, Ana; Garbaccio, Sergio; Cataldi, Angel; Caimi, Karina & Zumárraga, Martín

Abstract

Mycobacterium bovis check for this species in other resources is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.

Keywords
bovine TB; Mycobacterium bovis; spoligotype; VNTR

 
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