search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 3, 2014, pp. 324-329
Bioline Code: oc14044
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 3, 2014, pp. 324-329

 en Anti- Mycobacterium tuberculosis check for this species in other resources activity and cytotoxicity of Calophyllum brasiliense check for this species in other resources Cambess (Clusiaceae)
Pires, Claudia Terencio Agostinho; Brenzan, Mislaine Adriana; de Lima Scodro, Regiane Bertin; Cortez, Diógenes Aparício Garcia; Lopes, Luciana Dias Ghiraldi; Siqueria, Vera Lucia Dias & Cardoso, Rosilene Fressatti

Abstract

We evaluated the in vitro anti- Mycobacterium tuberculosis check for this species in other resources activity and the cytotoxicity of dichloromethane extract and pure compounds from the leaves of Calophyllum brasiliense check for this species in other resources . Purification of the dichloromethane extract yielded the pure compounds (-) mammea A/BB (1), (-) mammea B/BB (2) and amentoflavone (3). The compound structures were elucidated on the basis of spectroscopic and spectrometric data. The contents of bioactive compounds in the extracts were quantified using high performance liquid chromatography coupled to an ultraviolet detector. The anti-M. tuberculosis activity of the extracts and the pure compounds was evaluated using a resazurin microtitre assay plate. The cytotoxicity assay was performed in J774G.8 macrophages using the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colourimetric method. The quantification of the dichloromethane extract showed (1) and (2) at concentrations of 31.86 ± 2.6 and 8.24 ± 1.1 μg/mg of extract, respectively. The dichloromethane and aqueous extracts showed anti-M. tuberculosis H37Rv activity of 62.5 and 125 μg/mL, respectively. Coumarins (1) and (2) showed minimal inhibitory concentration ranges of 31.2 and 62.5 μg/mL against M. tuberculosis H37Rv and clinical isolates. Compound (3) showed no activity against M. tuberculosis H37Rv. The selectivity index ranged from 0.59-1.06. We report the activity of the extracts and coumarins from the leaves of C. brasiliense against M. tuberculosis.

Keywords
Mycobacterium tuberculosis; Calophyllum brasiliense; coumarin; cytotoxicity; 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide

 
© Copyright 2014 - Memórias do Instituto Oswaldo Cruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil