We evaluated the in vitro anti-
Mycobacterium tuberculosis
activity and the cytotoxicity of dichloromethane extract and pure compounds from the leaves of
Calophyllum brasiliense
. Purification of the dichloromethane extract yielded the pure compounds (-) mammea A/BB (1), (-) mammea B/BB (2) and amentoflavone (3). The compound structures were elucidated on the basis of spectroscopic and spectrometric data. The contents of bioactive compounds in the extracts were quantified using high performance liquid chromatography coupled to an ultraviolet detector. The anti-
M. tuberculosis activity of the extracts and the pure compounds was evaluated using a resazurin microtitre assay plate. The cytotoxicity assay was performed in J774G.8 macrophages using the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colourimetric method. The quantification of the dichloromethane extract showed (1) and (2) at concentrations of 31.86 ± 2.6 and 8.24 ± 1.1 μg/mg of extract, respectively. The dichloromethane and aqueous extracts showed anti-
M. tuberculosis H
37Rv activity of 62.5 and 125 μg/mL, respectively. Coumarins (1) and (2) showed minimal inhibitory concentration ranges of 31.2 and 62.5 μg/mL against
M. tuberculosis H
37Rv and clinical isolates. Compound (3) showed no activity against
M. tuberculosis H
37Rv. The selectivity index ranged from 0.59-1.06. We report the activity of the extracts and coumarins from the leaves of
C. brasiliense against
M. tuberculosis.
