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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 3, 2014, pp. 352-355
Bioline Code: oc14049
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 3, 2014, pp. 352-355

 en Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
de Campos, Karla Lopes Mandu; Machado, Ana Paula; de Almeida, Flávia Gatto; Bonin, Camila Mareti; Prata, Thiago Theodoro Martins; Almeida, Larissa Zatorre; Padovani, Cacilda Tezelli Junqueira; Ferreira, Alda Maria Teixeira; Fernandes, Carlos Eurico dos Santos & Tozetti, Inês Aparecida

Abstract

Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using type-specific PCR. HPV DNA detection showed good concordance between self and clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30% women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV was detected at a higher frequency in self-collected; however, HR-HPV types were more frequently identified in clinician-collected samples than in self-collected samples. HPV infections of multiple types were detected in 20.5% of clinician-collected samples and 15.5% of self-collected samples. In this study, we demonstrated that the HPV DNA detection rate in self-collected samples has good agreement with that of clinician-collected samples. Self-collected sampling, as a primary prevention strategy in countries with few resources, could be effective for identifying cases of HR-HPV, being more acceptable. The use of this method would enhance the coverage of screening programs for cervical cancer.

Keywords
human papillomavirus; self-collected; clinician-collected; PCR

 
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