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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 4, 2014, pp. 408-413
Bioline Code: oc14061
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 4, 2014, pp. 408-413

 en Oxidative stress enhances the expression of sulfur assimilation genes: preliminary insights on the Enterococcus faecalis check for this species in other resources iron-sulfur cluster machinery regulation
Riboldi, Gustavo Pelicioli; Bierhals, Christine Garcia; de Mattos, Eduardo Preusser; Frazzon, Ana Paula Guedes; d‘Azevedo, Pedro Alves & Frazzon, Jeverson

Abstract

The Firmicutes bacteria participate extensively in virulence and pathological processes. Enterococcus faecalis check for this species in other resources is a commensal microorganism; however, it is also a pathogenic bacterium mainly associated with nosocomial infections in immunocompromised patients. Iron-sulfur [Fe-S] clusters are inorganic prosthetic groups involved in diverse biological processes, whose in vivo formation requires several specific protein machineries. Escherichia coli check for this species in other resources is one of the most frequently studied microorganisms regarding [Fe-S] cluster biogenesis and encodes the iron-sulfur cluster and sulfur assimilation systems. In Firmicutes species, a unique operon composed of the sufCDSUB genes is responsible for [Fe-S] cluster biogenesis. The aim of this study was to investigate the potential of the E. faecalis sufCDSUB system in the [Fe-S] cluster assembly using oxidative stress and iron depletion as adverse growth conditions. Quantitative real-time polymerase chain reaction demonstrated, for the first time, that Gram-positive bacteria possess an OxyR component responsive to oxidative stress conditions, as fully described for E. coli models. Likewise, strong expression of the sufCDSUB genes was observed in low concentrations of hydrogen peroxide, indicating that the lowest concentration of oxygen free radicals inside cells, known to be highly damaging to [Fe-S] clusters, is sufficient to trigger the transcriptional machinery for prompt replacement of [Fe-S] clusters.

Keywords
suf operon; [Fe-S] cluster assembly; Firmicutes; oxidative stress; iron depletion

 
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