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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 109, No. 4, 2014, pp. 459-465
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Bioline Code: oc14069
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 4, 2014, pp. 459-465
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Sympathetic glial cells and macrophages develop different responses to Trypanosoma cruzi infection or lipopolysaccharide stimulation
de Almeida-Leite, Camila Megale; Silva, Isabel Cristina Costa; da Cunha Galvão, Lúcia Maria & Arantes, Rosa Maria Esteves
Abstract
Nitric oxide (NO) participates in neuronal lesions in the digestive form of Chagas disease and the proximity of
parasitised glial cells and neurons in damaged myenteric ganglia is a frequent finding. Glial cells have crucial roles
in many neuropathological situations and are potential sources of NO. Here, we investigate peripheral glial cell response
to Trypanosoma cruzi infection to clarify the role of these cells in the neuronal lesion pathogenesis of Chagas
disease. We used primary glial cell cultures from superior cervical ganglion to investigate cell activation and NO
production after T. cruzi infection or lipopolysaccharide (LPS) exposure in comparison to peritoneal macrophages.
T. cruzi infection was greater in glial cells, despite similar levels of NO production in both cell types. Glial cells
responded similarly to T. cruzi and LPS, but were less responsive to LPS than macrophages were. Our observations
contribute to the understanding of Chagas disease pathogenesis, as based on the high susceptibility of autonomic
glial cells to T. cruzi infection with subsequent NO production. Moreover, our findings will facilitate future research
into the immune responses and activation mechanisms of peripheral glial cells, which are important for understanding
the paradoxical responses of this cell type in neuronal lesions and neuroprotection.
Keywords
parasite; nitric oxide; superior cervical ganglion; glial cell culture
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