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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 4, 2014, pp. 466-472
Bioline Code: oc14070
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 4, 2014, pp. 466-472

 en Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism
Bounamous, Azzedine; Lehrter, Véronique; Hadj-Henni, Leila; Delecolle, Jean-Claude & Depaquit, Jérôme

Abstract

A total of 131 phlebotomine Algerian sandflies have been processed in the present study. They belong to the species Phlebotomus bergeroti check for this species in other resources , Phlebotomus alexandri check for this species in other resources , Phlebotomus sergenti check for this species in other resources , Phlebotomus chabaudi check for this species in other resources , Phlebotomus riouxi check for this species in other resources , Phlebotomus perniciosus check for this species in other resources , Phlebotomus longicuspis check for this species in other resources , Phlebotomus perfiliewi check for this species in other resources , Phlebotomus ariasi check for this species in other resources , Phlebotomus chadlii check for this species in other resources , Sergentomyia fallax check for this species in other resources , Sergentomyia minuta check for this species in other resources , Sergentomyia antennata check for this species in other resources , Sergentomyia schwetzi check for this species in other resources , Sergentomyia clydei check for this species in other resources , Sergentomyia christophersi check for this species in other resources and Grassomyia dreyfussi check for this species in other resources . They have been characterised by sequencing of a part of the cytochrome b (cyt b), t RNA serine and NADH1 on the one hand and of the cytochrome C oxidase I of the mitochondrial DNA (mtDNA) on the other hand. Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa. We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker. These method is not interesting to identify sandflies all over the Mediterranean Basin.

Keywords
Algeria; mtDNA; PCR-RFLP; Phlebotomus sergenti

 
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