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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 4, 2014, pp. 506-510
Bioline Code: oc14080
Full paper language: English
Document type: View Point
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 4, 2014, pp. 506-510

 en Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus
Luz, Sérgio Luiz Bessa; Crainey, James Lee; Shelley, Anthony John & Rubio, Jose Miguel


In a recent issue of Memórias do Instituto Oswaldo Cruz, published in Rio de Janeiro in February 2014 (109: 87-92), Adami et al. have published a survey reporting Mansonella parasite prevalence in the Amazon Region. This report makes a useful contribution to the existing knowledge of filarial parasite distribution within the Amazon area, parasite prevalence rates in relation to age and occupation and provides observations on the possible clinical impact of Mansonella ozzardi. Their publication also provides an account of what appears to be a novel ELISA that has recently been used in the Simuliidae and Onchocerciasis Laboratory of the Oswaldo Cruz Institute, Rio de Janeiro, Brazil. We are concerned that the publication of this ELISA may have created an excessively positive impression of the effectiveness of the onchocerciasis recrudescence serological surveillance tools that are presently available for use in the Amazonia onchocerciasis focus. In this letter we have, thus, sought to highlight some of the limitations of this ELISA and suggest how continuing insecurities concerning the detection of antibodies to Onchocerca volvulus within the Amazonia onchocerciasis focus might be minimised.

Amazonia; ELISA; Ov16; onchocerciasis; Mansonella ozzardi; recrudescence

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