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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 6, 2014, pp. 820-823
Bioline Code: oc14120
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 6, 2014, pp. 820-823

 en Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
Zuchi, Nayara; de Silva Heinen, Letícia Borges; dos Santos, Marcelo Adriano Mendes; Pereira, Fernanda Carla & Slhessarenko, Renata Dezengrini

Abstract

Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. MAYV was detected in 15 (2.5%) of 604 patients. Twelve were co-infected with dengue virus 4, which was isolated from 10 patients. The molecular detection of MAYV in dengue-suspected patients suggests that other arboviruses may be silently circulating during dengue outbreaks in Brazil.

Keywords
Mayaro virus; dengue virus 4; Mato Grosso

 
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