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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 7, 2014, pp. 871-878
Bioline Code: oc14129
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 7, 2014, pp. 871-878

 en Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis check for this species in other resources isolated from blood cultures
Pinheiro, Luiza; Brito, Carla Ivo; Pereira, Valéria Cataneli; de Oliveira, Adilson; Camargo, Carlos Henrique & da Cunha, Maria de Lourdes Ribeiro de Souza

Abstract

This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis check for this species in other resources isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1 , including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1 . The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment.

Keywords
Staphylococcus epidermidis; icaADBC; mecA; SCCmec; vancomycin; MIC

 
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