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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 7, 2014, pp. 944-947
Bioline Code: oc14139
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 7, 2014, pp. 944-947

 en Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
Barbieri, Raquel Rodrigues; Sales, Anna Maria; Illarramendi, Ximena; Moraes, Milton Ozório; da Costa Nery, José Augusto; Moreira, Suelen Justo Maria; Sarno, Euzenir Nunes; de Miranda Machado, Alice & Bozza, Fernando Augusto

Abstract

The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae check for this species in other resources -specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.

Keywords
paucibacillary leprosy; diagnosis; quantitative polymerase chain reaction

 
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