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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 109, No. 8, 2014, pp. 1078-1080
Bioline Code: oc14162
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 109, No. 8, 2014, pp. 1078-1080

 en Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
Barbosa, Joana; Rodrigues, Acácio Gonçalves; Pérez, Maria José & Pina-Vaz, Cidália

Abstract

Giardia duodenalis. (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (105/mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.

Keywords
Giardia duodenalis; flow cytometry; susceptibility

 
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