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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 1, 2015, pp. 65-74
Bioline Code: oc15004
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 1, 2015, pp. 65-74

 en Bioactive endophytic fungi isolated from Caesalpinia echinata check for this species in other resources Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium check for this species in other resources sp.
Campos, Fernanda Fraga; Junior, Policarpo A. Sales; Romanha, Alvaro José; Araújo, Márcio S.S.; Siqueira, Ezequias P.; Resende, Jarbas M.; Alves, Tânia M.A.; Martins-Filho, Olindo A.; dos Santos, Vera Lúcia; Rosa, Carlos A.; Zani, Carlos L. & Cota, Betania Barros

Abstract

Aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native Brazilian tree Caesalpinia echinata check for this species in other resources Lam. (Fabaceae). We tested their ethyl acetate extracts in several in vitro assays. The organic extracts from three isolates showed antibacterial activity against Staphylococcus aureus check for this species in other resources and Escherichia coli check for this species in other resources [minimal inhibitory concentration (MIC) 32-64 μg/mL]. One isolate inhibited the growth of Salmonella typhimurium check for this species in other resources (MIC 64 μg/mL) and two isolates inhibited the growth of Klebsiella oxytoca check for this species in other resources (MIC 64 μg/mL), Candida albicans check for this species in other resources and Candida tropicalis check for this species in other resources (MIC 64-128 μg/mL). Fourteen extracts at a concentration of 20 μg/mL showed antitumour activities against human breast cancer and human renal cancer cells, while two isolates showed anti-tumour activities against human melanoma cancer cells. Six extracts were able to reduce the proliferation of human peripheral blood mononuclear cells, indicating some degree of selective toxicity. Four isolates were able to inhibit Leishmania (Leishmania) amazonensis and one isolate inhibited Trypanosoma cruzi by at least 40% at 20 μg/mL. The trypanocidal extract obtained from Fusarium check for this species in other resources sp. [KF611679] culture was subjected to bioguided fractionation, which revealed beauvericin as the compound responsible for the observed toxicity of Fusarium sp. to T. cruzi. This depsipeptide showed a half maximal inhibitory concentration of 1.9 μg/mL (2.43 μM) in a T. cruzi cellular culture assay.

Keywords
endophytic fungi; bioactive; Caesalpinia echinata Lam.; Fabaceae; Trypanosoma cruzi; beauvericin

 
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