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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 1, 2015, pp. 106-113
Bioline Code: oc15009
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 1, 2015, pp. 106-113

 en Therapeutic switching: from antidermatophytic essential oils to new leishmanicidal products
Houël, Emeline; Gonzalez, German; Bessière, Jean-Marie; Odonne, Guillaume; Eparvier, Véronique; Deharo, Eric & Stien, Didier

Abstract

This study examined whether the antidermatophytic activity of essential oils (EOs) can be used as an indicator for the discovery of active natural products against Leishmania amazonensis. The aerial parts of seven plants were hydrodistilled. Using broth microdilution techniques, the obtained EOs were tested against three strains of dermatophytes ( Trichophyton mentagrophytes check for this species in other resources , Microsporum gypseum check for this species in other resources and Microsporum canis check for this species in other resources ). To compare the EOs antifungal and antiparasitic effects, the EOs activities against axenic amastigotes of L. amazonensis were concurrently evaluated. For the most promising EOs, their antileishmanial activities against parasites infecting peritoneal macrophages of BALB/c mice were measured. The most interesting antifungal candidates were the EOs from Cymbopogon citratus check for this species in other resources , Otacanthus azureus check for this species in other resources and Protium heptaphyllum check for this species in other resources , whereas O. azureus, Piper hispidum and P. heptaphyllum EOs exhibited the lowest 50% inhibitory concentration (IC50) values against axenic amastigotes, thus revealing a certain correspondence between both activities. The P. hispidum EO was identified as the most promising product in the results from the infected macrophages model (IC50: 4.7 μg/mL, safety index: 8). The most abundant compounds found in this EO were sesquiterpenes, notably curzerene and furanodiene. Eventually, the evaluation of the antidermatophytic activity of EOs appears to be an efficient method for identifying new potential drugs for the treatment of L. amazonensis.

Keywords
therapeutic switching; antifungal agents; antiparasitic agents; Leishmania; peritoneal macrophages; sesquiterpenes

 
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