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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 2, 2015, pp. 201-208
Bioline Code: oc15024
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 2, 2015, pp. 201-208

 en Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis check for this species in other resources
de Brum Vieira, Patrícia; Silva, Nícolas Luiz Feijó; Kist, Luiza Wilges; de Oliveira, Giovanna Medeiros Tavares; Bogo, Maurício Reis; De Carli, Geraldo Atillio; Macedo, Alexandre José & Tasca, Tiana

Abstract

Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5’-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis check for this species in other resources . Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalis isolates from female and male patients were evaluated. Iron from different sources sustained T. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5’-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron.

Keywords
Trichomonas vaginalis; iron; nucleoside triphosphate diphosphohydrolase; ecto-5’-nucleotidase; nucleotide hydrolysis

 
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