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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 2, 2015, pp. 272-274
Bioline Code: oc15036
Full paper language: English
Document type: Editorial
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 2, 2015, pp. 272-274

 en Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
de Paula, Fabiana Martins; de Mello Malta, Fernanda; Marques, Priscilla Duarte; Sitta, Renata Barnabé; Pinho, João Renato Rebello; Gryschek, Ronaldo César Borges & Chieffi, Pedro Paulo

Abstract

This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis check for this species in other resources (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.

Keywords
Strongyloides stercoralis; molecular diagnosis; conventional PCR; real-time PCR

 
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