Leptospirosis is a re-emerging zoonotic disease all over the world, important in tropical and subtropical areas. A
majority of leptospirosis infected patients present as subclinical or mild disease while 5-10% may develop severe infection
requiring hospitalisation and critical care. It is possible that several factors, such as the infecting serovar, level
of leptospiraemia, host genetic factors and host immune response, may be important in predisposition towards severe
disease. Different Leptospira
strains circulate in different geographical regions contributing to variable disease severity.
Therefore, it is important to investigate the circulating strains at geographical locations during each outbreak for
epidemiological studies and to support the clinical management of the patients. In this study immunochromatography,
microscopic agglutination test and polymerase chain reaction were used to diagnose leptospirosis. Further restriction
fragment length polymorphism and DNA sequencing methods were used to identify the circulating strains in two selected
geographical regions of Sri Lanka. Leptospira interrogans
, Leptospira borgpetersenii
and Leptospira kirschneri
strains were identified to be circulating in western and southern provinces. L. interrogans
was the predominant species
circulating in western and southern provinces in 2013 and its presence was mainly associated with renal failure.