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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 4, 2015, pp. 485-491
Bioline Code: oc15061
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 4, 2015, pp. 485-491

 en Molecular characterisation and disease severity of leptospirosis in Sri Lanka
Bandara, Kanchana Kumari; Weerasekera, Manjula; Gunasekara, Chinthika P.; Ranasinghe, Nilantha; Marasinghe, Chamil & Fernando, Neluka

Abstract

Leptospirosis is a re-emerging zoonotic disease all over the world, important in tropical and subtropical areas. A majority of leptospirosis infected patients present as subclinical or mild disease while 5-10% may develop severe infection requiring hospitalisation and critical care. It is possible that several factors, such as the infecting serovar, level of leptospiraemia, host genetic factors and host immune response, may be important in predisposition towards severe disease. Different Leptospira check for this species in other resources strains circulate in different geographical regions contributing to variable disease severity. Therefore, it is important to investigate the circulating strains at geographical locations during each outbreak for epidemiological studies and to support the clinical management of the patients. In this study immunochromatography, microscopic agglutination test and polymerase chain reaction were used to diagnose leptospirosis. Further restriction fragment length polymorphism and DNA sequencing methods were used to identify the circulating strains in two selected geographical regions of Sri Lanka. Leptospira interrogans check for this species in other resources , Leptospira borgpetersenii check for this species in other resources and Leptospira kirschneri check for this species in other resources strains were identified to be circulating in western and southern provinces. L. interrogans was the predominant species circulating in western and southern provinces in 2013 and its presence was mainly associated with renal failure.

Keywords
Leptospira; molecular characterisation; Sri Lanka

 
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