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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 5, 2015, pp. 662-668
Bioline Code: oc15089
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 5, 2015, pp. 662-668

 en Corynebacterium diphtheriae check for this species in other resources putative tellurite-resistance protein (CDCE8392_0813) contributes to the intracellular survival in human epithelial cells and lethality of Caenorhabditis elegans check for this species in other resources
dos Santos, Louisy Sanches; Antunes, Camila Azevedo; dos Santos, Cintia Silva; Pereira, José Augusto Adler; Sabbadini, Priscila Soares; de Luna, Maria das Graças; Azevedo, Vasco; Júnior, Raphael Hirata; Burkovski, Andreas; Asad, Lídia Maria Buarque de Oliveira & Mattos-Guaraldi, Ana Luíza

Abstract

Corynebacterium diphtheriae check for this species in other resources , the aetiologic agent of diphtheria, also represents a global medical challenge because of the existence of invasive strains as causative agents of systemic infections. Although tellurite (TeO32−) is toxic to most microorganisms, TeO32−-resistant bacteria, including C. diphtheriae, exist in nature. The presence of TeO32−-resistance (TeR) determinants in pathogenic bacteria might provide selective advantages in the natural environment. In the present study, we investigated the role of the putative TeR determinant (CDCE8392_813 gene) in the virulence attributes of diphtheria bacilli. The disruption of CDCE8392_0813 gene expression in the LDCIC_L1 mutant increased susceptibility to TeO32− and reactive oxygen species (hydrogen peroxide), but not to other antimicrobial agents. The LDCIC-L1 mutant also showed a decrease in both the lethality of Caenorhabditis elegans check for this species in other resources and the survival inside of human epithelial cells compared to wild-type strain. Conversely, the haemagglutinating activity and adherence to and formation of biofilms on different abiotic surfaces were not regulated through the CDCE8392_0813 gene. In conclusion, the CDCE8392_813 gene contributes to the TeR and pathogenic potential of C. diphtheriae.

Keywords
Corynebacterium diphtheriae; tellurite-resistance; CDCE8392_0813 protein; HEp-2 cells; Caenorhabditis elegans

 
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