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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 5, 2015, pp. 677-683
Bioline Code: oc15091
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 5, 2015, pp. 677-683

 en Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
Santos, Jefferson José da Silva; Magalhães, Tereza; Junior, José Valter Joaquim Silva; da Silva, Andréa Nazaré Monteiro Rangel; Cordeiro, Marli Tenório & Gil, Laura Helena Vega Gonzales

Abstract

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.

Keywords
infectious clone; yeast homologous recombination; flavivirus

 
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