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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 5, 2015, pp. 693-699
Bioline Code: oc15095
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 5, 2015, pp. 693-699

 en Trypanocidal, trichomonacidal and cytotoxic components of cultivated Artemisia absinthium check for this species in other resources Linnaeus (Asteraceae) essential oil
Martínez-Díaz, Rafael Alberto; Ibáñez-Escribano, Alexandra; Burillo, Jesús; de las Heras, Lorena; del Prado, Gema; Agulló-Ortuño, M. Teresa; Julio, Luis F. & González-Coloma, Azucena

Abstract

Artemisia absinthium check for this species in other resources is an aromatic and medicinal plant of ethnopharmacological interest and it has been widely studied. The use of A. absinthium based on the collection of wild populations can result in variable compositions of the extracts and essential oils (EOs). The aim of this paper is the identification of the active components of the vapour pressure (VP) EO from a selected and cultivated A. absinthium Spanish population (T2-11) against two parasitic protozoa with different metabolic pathways: Trypanosoma cruzi and Trichomonas vaginalis. VP showed activity on both parasites at the highest concentrations. The chromatographic fractionation of the VP T2-11 resulted in nine fractions (VLC1-9). The chemical composition of the fractions and the antiparasitic effects of fractions and their main compounds suggest that the activity of the VP is related with the presence of trans-caryophyllene and dihydrochamazulene (main components of fractions VLC1 and VLC2 respectively). Additionally, the cytotoxicity of VP and fractions has been tested on several tumour and no tumour human cell lines. Fractions VLC1 and VLC2 were not cytotoxic against the nontumoural cell line HS5, suggesting selective antiparasitic activity for these two fractions. The VP and fractions inhibited the growth of human tumour cell lines in a dose-dependent manner.

Keywords
Artemisia absinthium; essential oil; activity; Trypanosoma cruzi; Trichomonas vaginalis; cytotoxicity

 
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