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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 110, No. 6, 2015, pp. 719-725
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Bioline Code: oc15097
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 6, 2015, pp. 719-725
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Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia
Hoyos-López, Richard; Soto, Sandra Uribe; Rúa-Uribe, Guillermo & Gallego-Gómez, Juan Carlos
Abstract
Saint Louis encephalitis virus (SLEV) is a member of the Japanese-encephalitis virus serocomplex of the genus
Flavivirus. SLEV is broadly distributed in the Americas and the Caribbean Islands, where it is usually transmitted
by mosquitoes of the genus Culex and primarily to birds and mammalian-hosts. Humans are occasionally infected
by the virus and are dead-end hosts. SLEV causes encephalitis in temperate regions, while in tropical regions of
the Americas, several human cases and a wide biological diversity of SLEV-strains have been reported. The phylogenetic
analysis of the envelope (E) protein genes indicated eight-genotypes of SLEV with geographic overlap.
The present paper describes the genotyping of two SLEV viruses detected in mosquito-pools collected in northern
Colombia (department of Cordoba). We used reverse transcription-polymerase chain reaction to amplify a fragment
of the E-gene to confirm the virus identity and complete E-gene sequencing for phylogenetic analysis and genotyping
of the two-SLEV viruses found circulating in Córdoba. This is the first report of SLEV genotype IV in Colombia
(Córdoba) in mosquitoes from a region of human inhabitation, implicating the risk of human disease due to SLEV infection.
Physicians should consider SLEV as a possible aetiology for undiagnosed febrile and neurologic syndromes
among their patients who report exposure to mosquito-bites.
Keywords
Saint Louis encephalitis virus; flavivirus; genotype; Colombia
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