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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 6, 2015, pp. 745-754
Bioline Code: oc15101
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 6, 2015, pp. 745-754

 en Detection of Oropouche virus segment S in patients and in Culex quinquefasciatus check for this species in other resources in the state of Mato Grosso, Brazil
Cardoso, Belgath Fernandes; Serra, Otacília Pereira; Heinen, Letícia Borges da Silva; Zuchi, Nayara; de Souza, Victor Costa; Naveca, Felipe Gomes; dos Santos, Marcelo Adriano Mendes & Slhessarenko, Renata Dezengrini

Abstract

This study aimed to investigate the circulation of Orthobunyavirus species in the state of Mato Grosso (MT) Brazil. During a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female Culex quinquefasciatus check for this species in other resources captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment S of the Simbu serogroup followed by nucleotide sequencing and virus isolation in Vero cells. Patients (5/529; 0.9%) from Cuiabá (n = 3), Várzea Grande (n = 1) and Nova Mutum (n = 1) municipalities were positive for the S segment of Oropouche virus (OROV). Additionally, eight/387 Cx. quinquefasciatus pools were positive for the segment, with a minimum infection rate of 2.3. Phylogenetic analysis indicated that all the samples belong to the subgenotype Ia, presenting high homology with OROV strains obtained from humans and animals in the Brazilian Amazon. The present paper reports the first detection of an Orthobunyavirus, possibly OROV, in patients and in Cx. quinquefasciatus mosquitoes in MT. This finding reinforces the notion that arboviruses frequently reported in the Amazon Region circulate sporadically in MT during dengue outbreaks.

Keywords
OROV; Orthobunyavirus; Mato Grosso; mosquitoes; phylogenetic analysis

 
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