|
Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 110, No. 6, 2015, pp. 786-792
|
Bioline Code: oc15106
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 6, 2015, pp. 786-792
en |
Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010
Bertol, Jéssica Wildgrube; Fregolente, Maria Clara Duarte; Caruzo, Thabata Alessandra Ramos; da Silva, Márcio José; Munford, Veridiana; Sáfadi, Marco Aurélio Palazzi; Rácz, Maria Lucia & Gatti, Maria Silvia Viccari
Abstract
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins
(VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral
enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic
diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse
transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed
genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage
between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters,
with grouping of most strains by their genotype and collection year, and most strains from SP were clustered
together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many
variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate
host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating
the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new
genotype combinations emerge through reassortment and independent segregation.
Keywords
human rotavirus; NSP4 genotypes; phylogenetic analysis; VP4/VP7/NSP4 linkage; Brazil
|
|
© Copyright 2015 - Memórias do Instituto Oswaldo Cruz Alternative site location: http://memorias.ioc.fiocruz.br
|
|