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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 7, 2015, pp. 906-913
Bioline Code: oc15124
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 7, 2015, pp. 906-913

 en Aspidosperma check for this species in other resources (Apocynaceae) plant cytotoxicity and activity towards malaria parasites. Part II: experimental studies with Aspidosperma ramiflorum check for this species in other resources in vivo and in vitro
Aguiar, Anna C.C.; Cunha, Ananda C.; Penna Ceravolo, Isabela; Correia Gonçalves, Regina A.; Oliveira, Arildo J.B. & Ursine Krettli, Antoniana

Abstract

Several species of Aspidosperma check for this species in other resources plants are used to treat diseases in the tropics, including Aspidosperma ramiflorum check for this species in other resources , which acts against leishmaniasis, an activity that is experimentally confirmed. The species, known as guatambuyellow, yellow peroba, coffee-peroba and matiambu, grows in the Atlantic Forest of Brazil in the South to the Southeast regions. Through a guided biofractionation of A. ramiflorum extracts, the plant activity against Plasmodium falciparum was evaluated in vitro for toxicity towards human hepatoma G2 cells, normal monkey kidney cells and nonimmortalised human monocytes isolated from peripheral blood. Six of the seven extracts tested were active at low doses (half-maximal drug inhibitory concentration < 3.8 μg/mL); the aqueous extract was inactive. Overall, the plant extracts and the purified compounds displayed low toxicity in vitro. A nonsoluble extract fraction and one purified alkaloid isositsirikine (compound 5) displayed high selectivity indexes (SI) (= 56 and 113, respectively), whereas compounds 2 and 3 were toxic (SI < 10). The structure, activity and low toxicity of isositsirikine in vitro are described here for the first time in A. ramiflorum, but only the neutral and precipitate plant fractions were tested for activity, which caused up to 53% parasitaemia inhibition of Plasmodium berghei in mice with blood-induced malaria. This plant species is likely to be useful in the further development of an antimalarial drug, but its pharmacological evaluation is still required.

Keywords
Aspidosperma ramiflorum; ethnopharmacology; antimalarials; P. falciparum and medicinal plants

 
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