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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 110, No. 8, 2015, pp. 989-995
Bioline Code: oc15134
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 8, 2015, pp. 989-995

 en DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
Forster, Karine M.; Hartwig, Daiane D.; Oliveira, Thaís L.; Bacelo, Kátia L.; Schuch, Rodrigo; Amaral, Marta G. & Dellagostin, Odir A.

Abstract

Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira check for this species in other resources genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA primeprotein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain of Leptospira interrogans check for this species in other resources , the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.

Keywords
leptospirosis; Leptospira; LigBrep; subunit vaccine; DNA vaccine; prime-boost

 
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